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(a, b) Intracellular replication and infectivity of C. <t>trachomatis</t> is inhibited upon loss of ARF4 in HeLa cells. (a) Quantitative PCR analysis of cells infected with C. trachomatis shows a significant decrease of bacterial genomes present after 48 hours p<0.05 (one-way ANOVA) but not after 12 hours in ARF4 KD cells relative to controls. Four wells were analyzed, and the experiment was performed twice. Bar graphs depict mean values, and error bars correspond to S.D.
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(a, b) Intracellular replication and infectivity of C. trachomatis is inhibited upon loss of ARF4 in HeLa cells. (a) Quantitative PCR analysis of cells infected with C. trachomatis shows a significant decrease of bacterial genomes present after 48 hours p<0.05 (one-way ANOVA) but not after 12 hours in ARF4 KD cells relative to controls. Four wells were analyzed, and the experiment was performed twice. Bar graphs depict mean values, and error bars correspond to S.D.

Journal: Nature cell biology

Article Title: A Luman/CREB3-ADP-ribosylation factor 4 (ARF4) signaling pathway mediates the response to Golgi stress and susceptibility to pathogens

doi: 10.1038/ncb2865

Figure Lengend Snippet: (a, b) Intracellular replication and infectivity of C. trachomatis is inhibited upon loss of ARF4 in HeLa cells. (a) Quantitative PCR analysis of cells infected with C. trachomatis shows a significant decrease of bacterial genomes present after 48 hours p<0.05 (one-way ANOVA) but not after 12 hours in ARF4 KD cells relative to controls. Four wells were analyzed, and the experiment was performed twice. Bar graphs depict mean values, and error bars correspond to S.D.

Article Snippet: HSP60 (1:500, sc-57840/clone A57-B9, Santa Cruz Biotechnology), rabbit anti-GRASP65/GORASP1 (1:1000, GTX112112, GeneTex), mouse anti-p84 (1:1000, GTX70220/clone 5E10, GeneTex), rabbit anti-GSK3β (1:1000, #9315/clone 27C10, Cell Signaling Technologies), rabbit anti-BIG1 (1:1000, A300-998A, Bethyl Laboratories), rabbit anti-BIG2 (1:1000, A301-004A, Bethyl Laboratories), mouse anti- C. trachomatis -MOMP (1:5 Fluorescein-conjugated; #30702, Biorad).

Techniques: Infection, Real-time Polymerase Chain Reaction